Optimizing Protein Separations with Agilent Weak Cation-Exchange Columns

Faster Separations Using Agilent Weak Cation Exchange Columns

Optimizing protein separations with cation exchange chromatography using Agilent Buffer Advisor

This Technical Overview shows that the Agilent Buffer Advisor software in combination with the Agilent 1260 Infi nity Bio-inert Quaternary LC System is an ideal solution for automated protein separation by ionic strength gradients. Usually, pH scouting using premixed two-component gradients is time-consuming and workintensive. Dynamically mixed four-component gradients calculated by the Buffer ...

Optimizing protein separations with cation exchange chromatography using Agilent Buffer Advisor Protein separation with the Agilent 1260 Infinity Bio‐inert Quaternary LC System

This Technical Overview shows that the Agilent Buffer Advisor software in com‐ bination with the Agilent 1260 Infinity Bio‐inert Quaternary LC System is an ideal solution for automated protein separation by ionic strength gradients. Usually, pH scouting using premixed two‐component gradients is time‐consuming and work‐ intensive. Dynamically mixed four‐component gradients calculated by the Buff...

Separation of Monoclonal Antibodies by Weak Cation-Exchange Chromatography Using ProPac and ProSwift Columns

Monoclonal antibodies (MAbs) are currently developed by pharmaceutical and biotechnology companies for various therapeutic applications. MAbs undergo several post-translational modifications including oxidations, deamidations, lysine truncations, and glycan modifications. Manufacturing of MAbs and subsequent stability testing procedures involve routine analysis and monitoring of the impurities ...

Effects of Weak and Strong Cation Exchange Resins on Moisture Uptake Behavior of Ranitidine Hydrochloride

     The aim of the present research was to study the effects of weak cation exchange resins, polacrilex with exchangeable H+ and polacrillin potassium and strong cation exchange resin sodium polystyrene sulfonate on water uptake behavior of ranitidine hydrochloride. Drug resin complexes (DRC) were prepared and evaluated for the percentage of moisture gain when placed in a humidity c...